Selected MCQ

Current Question
In a cloning vector, what is the purpose of including an antibiotic resistance gene?
  • A. To replicate the plasmid in host cells
  • B. As a selectable marker to identify transformed cells
  • C. To transcribe the cloned gene at high levels
  • D. To initiate DNA replication
Correct Answer: B
Explanation:
The antibiotic resistance gene allows selection: only bacteria that have taken up the plasmid (and express resistance) will grow on antibiotic-containing media.
Related Question 1
In gene therapy using a viral vector, what is a major concern related to using retroviruses?
  • A. They cannot infect human cells
  • B. They only integrate into mitochondrial DNA
  • C. Insertional mutagenesis by random integration into the host genome
  • D. They cannot carry large genes
Correct Answer: C
Explanation:
Retroviral vectors integrate randomly into the host genome, risking insertional mutagenesis (disrupting host genes), which can cause cancer or other issues.
Related Question 2
Which viral vector carries a high risk of insertional mutagenesis due to genome integration?
  • A. Adenoviral vector
  • B. Retroviral vector
  • C. Adeno-associated viral vector
  • D. Liposome-based vector
Correct Answer: B
Explanation:
Retroviruses integrate their genome into the host DNA. This random insertion can disrupt host genes, increasing the risk of mutagenesis (used with caution in gene therapy).
Related Question 3
Alternative splicing occurs during gene expression at the level of:
  • A. DNA replication
  • B. Transcription initiation
  • C. Pre-mRNA processing (RNA splicing)
  • D. Protein folding
Correct Answer: C
Explanation:
Alternative splicing of pre-mRNA allows different exons to be joined or skipped, producing multiple mRNA variants from one gene during RNA processing.
Related Question 4
Which term describes genes that are located on the same chromosome and inherited together more often than by chance?
  • A. Linked genes
  • B. Epistatic genes
  • C. Polygenic genes
  • D. Independent genes
Correct Answer: A
Explanation:
Linked genes are near each other on the same chromosome. They tend to be inherited together, and their recombination frequency is less than 50%.
Related Question 5
Somatic cell nuclear transfer (SCNT) for reproductive cloning involves:
  • A. Injecting somatic cell DNA into a fertilized egg with its nucleus intact
  • B. Fusing a diploid somatic cell with an enucleated egg cell
  • C. Using only oocyte mitochondria from a donor
  • D. Cloning via binary fission
Correct Answer: B
Explanation:
SCNT (as in cloning Dolly the sheep) entails transferring the nucleus of an adult somatic cell into an enucleated oocyte, and then stimulating the resulting cell to divide as an embryo.