Selected MCQ

Current Question
Which of the following components is *not* required for a standard PCR reaction?
  • A. Template DNA
  • B. DNA polymerase
  • C. Primers
  • D. DNA ligase
Correct Answer: D
Explanation:
PCR requires a thermostable DNA polymerase, primers, dNTPs, and template DNA. DNA ligase is not needed because PCR does not splice DNA fragments.
Related Question 1
Lipid rafts in the plasma membrane are microdomains enriched in which of the following components?
  • A. Cholesterol and sphingolipids
  • B. Phosphatidylcholine
  • C. Triglycerides
  • D. Integral membrane proteins only
Correct Answer: A
Explanation:
Lipid rafts are rich in cholesterol and sphingolipids, which pack tightly, creating ordered membrane regions.
Related Question 2
Which of the following best describes polygenic inheritance?
  • A. One gene affects multiple traits (e.g., pleiotropy)
  • B. Many genes contribute to one trait (e.g., skin color)
  • C. A trait determined by genes only on the X chromosome
  • D. Inheritance of mitochondrial DNA
Correct Answer: B
Explanation:
Polygenic inheritance means multiple genes (often with additive effects) determine a single phenotype, such as height or skin color, producing continuous variation.
Related Question 3
Which of the following is NOT typically controlled during industrial fermentation in a bioreactor?
  • A. Temperature
  • B. pH
  • C. Oxygen concentration
  • D. DNA sequence of the microorganism
Correct Answer: D
Explanation:
A, B, and C (temperature, pH, oxygen) are critical parameters controlled in fermentation. The chosen strain fixes the DNA sequence and is not a controlled parameter during the run.
Related Question 4
What distinguishes reverse transcription PCR (RT-PCR) from standard PCR?
  • A. Use of DNA ligase
  • B. Amplification of RNA by first creating complementary DNA
  • C. Higher temperature cycles
  • D. Use of radioactive nucleotides
Correct Answer: B
Explanation:
RT-PCR involves reverse transcription of RNA into cDNA before PCR, enabling amplification of RNA targets.
Related Question 5
Which of the following techniques compares short tandem repeat (STR) regions among individuals for identification?
  • A. Western blot
  • B. DNA microarray
  • C. DNA fingerprinting
  • D. Northern blot
Correct Answer: C
Explanation:
DNA fingerprinting analyzes highly polymorphic regions, especially STRs, to distinguish individuals (used in forensics and paternity testing).